To streak plate and bacterial culture transfer using aseptic technique.

Introduction:
Aseptic technique is a laboratory technique that is required to transfer pure cultures and performed under sterile conditions to prevent contamination of the culture by unwanted microbes. Bacterial culture streaking allowed bacteria to reproduce on a culture medium in a controlled environment. The process streaked bacteria in four section on an agar plate and sealed with parafilm and allowed to incubate at 37°C temperature for about 16 to 18 hours. Bacterial culture streaking can be used to identify and isolate pure bacteria into individual colonies. Microbiologists use bacterial and other microbial culture streaking methods to identify microorganisms and to diagnose infection.

Materials:
Agar plate, bacteria culture slant, acetone burner, lighter, inoculating loop, parafilm and Digital constant temperature tank (water bath).

Methods:

1. Labeled the bottom of agar plate with name, date, culture name and labeled number 1, 2, 3, 4 counter clockwise, each across ¼ of the plate.
2. Lighted acetone burner with lighter, flamed the inoculating loop tip until it turned orange color, then passed the loop wire to flame, and let it cool down.
3. Opened the bacteria culture slant cap by right hand hold the inoculating loop and the cap of the culture slant while left hand hold and loosen the culture slant, passed the lip through the flame, and then dipped the loop into the culture slant to transfer the bacteria to a new agar plate, tighten the cap of the culture slant, then opened the lid of the agar plate enough for the loop in while other hand put the inoculating loop that had bacteria culture sample to section 1 labeled on the plate to transfer bacteria sample by streaked the agar surface lightly back and forth several time, then closed the plate, turned the culture plate to section 2 and flamed the loop at the same time, and allowed to cool down, then opened the lid plate, used the inoculating loop to streak on section 2 several times overlap the end of section 1, closed the plate, and turned the plate to section 3, and flamed the loop at the same time, and allowed to cool down, and used the same steps to finish streaking in section 3 and 4.
4. The different of section 4 only streaked one line overlap the end of section 3, and made sure did not touched other section, then flamed the loop and allowed to cool down and put it on the table. Flipped the culture plate and sealed with parafilm, opened the top cover of the water bath, put the bacteria culture plate upside down in the water bath, made sure had enough water, then closed the cover and set 37°C temperature, allowed to incubate about 16 to 18 hours.
5. Viewed the result to identify and isolated colonies from the bacteria culture slant.